Some Important Short Questions about Micro Techniques For MLT Students:
*Microscopy Techniques*
1. Brightfield Microscopy: Uses visible light to illuminate the sample.
2. Darkfield Microscopy: Uses scattered light to illuminate the sample.
3. Phase Contrast Microscopy: Uses differences in refractive index to create contrast.
4. Fluorescence Microscopy: Uses fluorescent dyes to illuminate the sample.
5. Electron Microscopy: Uses a beam of electrons to produce a high-resolution image.
*Staining Techniques*
1. Gram Staining: Differentiates bacteria into Gram-positive and Gram-negative cells.
2. Acid-Fast Staining: Identifies acid-fast bacteria such as Mycobacterium tuberculosis.
3. Endospore Staining: Identifies endospores in bacteria.
4. Capsule Staining: Identifies capsules in bacteria.
5. Flagella Staining: Identifies flagella in bacteria.
*Cultivation Techniques*
1. Broth Culture: Grow bacteria in a liquid medium.
2. Agar Plate Culture: Grow bacteria on a solid medium.
3. Slant Culture: Grow bacteria on a slanted agar surface.
4. Stab Culture: Grow bacteria in a deep agar medium.
5. Anaerobic Culture: Grow bacteria in the absence of oxygen.
*Identification Techniques*
1. Biochemical Tests: Identify bacteria based on their metabolic characteristics.
2. API (Analytical Profile Index) Identification: Identify bacteria using a commercial identification system.
3. PCR (Polymerase Chain Reaction) Identification: Identify bacteria using DNA amplification.
4. Sequencing Identification: Identify bacteria using DNA sequencing.
5. MALDI-TOF (Matrix-Assisted Laser Desorption/Ionization-Time of Flight) Identification: Identify bacteria using protein profiling.
*Safety Techniques*
1. Personal Protective Equipment (PPE): Wear gloves, lab coats, and eye protection.
2. Sterilization: Use autoclaves or other methods to sterilize equipment and supplies.
3. Disinfection: Use disinfectants to clean surfaces and equipment.
4. Waste Disposal: Properly dispose of biohazardous waste.
5. Emergency Procedures: Know what to do in case of an emergency.
